Food oral processing is a key stage for safe swallowing, during which
ingested foods are broken down through compression and shear by teeth and
tongue and then mixed with saliva to form a cohesive bolus. In this study,
we prepared three model bakery crackers: control that was made from wheat
flour and water, a shortening-added sample, and a WPI-added sample. The
three model crackers were fragmented into granules of ~1 mm for preparing
artificial boluses by subsequently impregnating the granules with an amount
of artificial saliva without amylase. The mechanical strength of the
saliva-absorbed granules was evaluated by a penetration test, and the bolus
rheology was characterized by strain-sweep and frequency-sweep measurements.
The cracker samples with added fat exhibited a brittle and weak structure
compared with the control, possibly due to the disruption of the gluten
network by shortening. In contrast, the WPI-added samples showed greater
mechanical strength than the control, which was attributed to the
reinforcement of the protein network by WPI. As a result, the fat-added
sample developed a sticky surface layer after absorbing 60% saliva, as the
granule surface partially broke down. This sticky layer promoted strong
inter-granular adhesion. However, the WPI-added samples showed weak adhesion
due to lubrication by unabsorbed saliva, as their firm structure limited
saliva penetration. With increasing saliva uptake, the mechanical strength
of all samples decreased, while the overall strength remained in the order
WPI-added > control > fat-added. The elastic modulus (G′) obtained
from rheological measurements also decreased with saliva uptake in all
samples. However, at 60% saliva, the fat-added samples showed a higher G′
than the control, despite their weaker individual granules, indicating that
adhesive interactions between granules contributed to the bolus elasticity.
In contrast, the WPI-added samples maintained higher elasticity than the
other samples at all saliva levels, reflecting their inherently greater
mechanical strength. In conclusion, our study clarified how the mechanical
strength of the granules and the adhesion formed between them after saliva
uptake influence bolus rheology. In addition, adjusting ingredient
composition can modulate these rheological properties, providing useful
insights for designing bakery products with improved texture and
swallowability.