Sustainable active film from chitosan (CS) incorporated with microalgae Haematococcus pluvialis extract (HPE) was developed. Pulsed electric field (PEF) with the field strength of 25 kV∙cm ^-1 and total specific energy of 200 kJ∙kg ^-1 was optimal to extract H. pluvialis by inducing the electroporation in the cytoplasmic membrane, resulting in improving the solvent-mediated extraction of the bioactive compounds, as evident by the analysis results of GC-MS and HPLC-MS. The active film was then fabricated by infusing CS with HPE or PEF-assisted HPE (PEF-HPE), and characterized for tensile strength, elongation, opacity, water vapor barrier and antioxidant activity. The optimal CS+PEF-HPE film was then used to wrap chicken fillet, in comparison to CS and CS+HPE. Chicken meat without film wrapping was used as control. All samples were stored at 4 °C and monitored for changes in color, total volatile basic nitrogen (TVB-N), trimethylamine (TMA), total viable counts (TVC) throughout the storage. Chicken breast wrapped with CS, CS+HPE and CS+PEF-HPE had significantly lower TVB-N, TMA, TVC than control samples during storage. The food preserving capability of the fabricated films increased in ascending order from CS, CS+HPE, to CS+PEF-HPE. These results indicated the fabricated CS film synergized with microalgae extract has potential as active packaging to preserve quality and extend shelf-life of chicken meat.