This study investigates the impact of various drying techniques on the physicochemical and functional properties of mucilage extracted from lemon basil ( Ocimum × africanum Lour) seeds. Mucilage samples, containing a solid content of 0.5–0.6 g per 100 g of solution, were obtained via ultrasonic-assisted extraction at 150 W, for 15 minutes, using a 1:40 solid-to-liquid ratio. It is hypothesized that the drying method influences the properties of rehydrated mucilage. The first method, called tray drying (TD), involved drying the extracted mucilage at 60 °C in a petri dish inside a forced-convection oven for 24 hours. The second method, known as drum drying (DD), was performed in a twin-row drum dryer, set at 130 °C and 10 rpm, with the liquid mucilage fed at a rate of 4 kg/h. The third method, called freeze-drying (FD), started by freezing a mucilage sample in a petri dish at -80 °C, followed by sublimation at -65 °C under a vacuum of 0.0001 Pa. The final method, known as spray drying (SP), was carried out in a lab-scale spray dryer using a 0.7-mm fixed nozzle, with an inlet temperature of 170°C, compressed air pressure of 4.0 MPa, and feed flow rate of 0.3 kg/h. All methods were evaluated based on their drying yield and moisture content to assess their drying efficiency. Since the SP produced fine particles, before measuring properties, the mucilage sheets from TD and FD, and the mucilage flakes from DD, were ground into powder with particle sizes less than 0.1 mm to normalize particle size effects. The physicochemical properties, including density, viscosity, and molecular weight distribution, were analyzed. Furthermore, the functional properties evaluated included water and oil holding capacities, emulsification capacity and stability, water solubility, color, water activity, dispersion stability, and moisture content.