Purpose: gelatin and pectin were selected to prepare composite hydrogels with interpenetrating polymer networks (IPN) structure. Such IPN hydrogels was suitable as scaffolds to support the cultivation of muscle stem cells. Methods: a series of IPN and semi-IPN hydrogels were prepared by using TGase and calcium salt as cross-linking agents.The hydrogel scaffolds were analyzed with hydration, structural, rheological and mechanical assessments. The cyto-compatibility of scaffolds was evaluated by the chicken muscle satellite cells (CMSCs). The characterization of cultured meats on IPN scaffolds was determined by the appearance, water distribution and storage stability. Results: In terms of physical and chemical properties, the IPN scaffolds had more excellent mechanical property and structural stability compared with semi-IPN scaffolds. It was shown that IPN hydrogels were favorable for CMSCs to form multinucleated myotubes and supported CMSCs to express relevant functional proteins. Meanwhile, it had the stronger pro-cell proliferation ability than semi-IPN hydrogels. The cultured meat within hydrogels as scaffold exhibited large myofibrils similar to chicken tissue and had the advantage of being more resistant to storage than chicken. Conclusion: this study provides a new strategy for the effective utilization of IPN hydrogels as scaffolds for cell cultured meat.